The Forest-CODEX

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The Forest-CODEX

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The Forest – As the lone survivor of a passenger jet crash, you find yourself in a mysterious forest battling to stay alive against a society of .
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The Forest – As the lone survivor of a passenger jet crash, you find yourself in a mysterious forest battling to stay alive against a society of .Bistability in gene expression observed with a highly sensitive fluorescence microscope.
One novel and elusive property of living organisms is the capability of bistability. This unique property in gene expression seems to be an evolutionary strategy to regulate the genetic network and to drive the cell to a certain fate when a certain condition is met. Currently, the operational definition of bistability and its demarcation from the other types of phenomena are under debate. Here we designed a reporter gene system to observe the bistability using a highly sensitive fluorescence microscope and a standard microscope. The reporter system was composed of the ß-lactamase gene (bla) under a LacZ promoter, followed by an internal ribosomal entry sequence (IRES) element and the green fluorescent protein (GFP). Measurements showed that only a small fraction (1.06%) of the population (∼105 cells) showed bistability when the cells were induced at the M(-) position. The bistable fraction increased to (14.3 ± 0.8)% in the presence of elevated glucose (10-500 mM). These observations indicated that bistability is a fundamental property of living organisms and a standard microscope can be used for its measurement.Electrotonic currents of retinal bipolar cells in the rabbit.
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