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Foto-Mosaik is a powerful and accurate tools for creating mosaic pictures (with pictures) and photographsÂ .Phosphatidylcholine metabolism during the early and late stages of human trophoblast cell differentiation.
The synthesis and distribution of phosphatidylcholine, a major constituent of trophoblast cell plasma membranes, was studied in human trophoblast cells after the addition of dibutyryl cyclic AMP (dbcAMP), theophylline, or, in the late stages of differentiation, after the addition of choriogonadotropin (hCG). Two biochemical approaches were used, the biosynthesis of choline 14C-phosphate, which is a key intermediate in the formation of phosphatidylcholine, and the incorporation of 14C-choline into total lipids. The addition of dbcAMP, theophylline, or hCG (1 IU/ml, 24 h in culture) had no effect on phosphatidylcholine biosynthesis. However, these agents caused a rapid and significant (p less than 0.01) increase in the incorporation of 14C-choline into phosphatidylcholine. The increase in phosphatidylcholine accumulation could be reached with relatively small doses of hCG and in the late stages of differentiation was significantly greater (p less than 0.001) than that observed in the cells in which differentiation was stimulated only by cAMP. These results suggest that human trophoblast cells undergo two stages of differentiation, with the first being characterised by an increase in the rate of phosphatidylcholine formation.Q:
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